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主营:亚磷酰安,DNA和RNA寡核苷酸的化学合成物
℡ 4000-520-616
℡ 4000-520-616
Glen research/UniCap Phosphoramidite/1kit/10-4410-20
产品编号:10-4410-20
市  场 价:¥8000.00
场      地:美国(厂家直采)
产品分类: 生化试剂>其他>>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$400.00
品      牌: Glen research
公      司:Glenresearch,inc
公司分类:
Glen research/UniCap Phosphoramidite/1kit/10-4410-20
商品介绍
Technical Documents
Description
Details
Intellectual Property
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Glen Report 17.13: UniCap Phosphoramidite, AN alternative to acetic anhydride capping

Glen Report 30.1: Technical Snippets


Description

The phosphoramidite of diethylene glycol monoethyl ether, UniCap, is the basis for an alternative capping reagent.To use UniCap as a capping amidite on the Expedite 8909 or AB synthesizers, dilute it to the standard amidite concentration and place the vial in position 5 on the instrument.Cycles can be modified by adding coupling steps for amidite reservoir 5 after the last column coupling step.The standard capping steps can be left out of the cycle.UniCap Phosphoramidite was originally developed for oligo synthesis on the surface of chips and is the capping reagent of choice for this application.

Details

Usage

  • Coupling: For capping, add as a second coupling step after base coupling using standard coupling conditions.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
DiluentAnhydrous Acetonitrile
StorageFreezer storage, -10 to -30°C, dry
Stability7 days

Intellectual Property

This product is supplied under license.

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
10-4410-020.25 g.25grams7.48236141.688.564.3647.211.8
10-4410-050.5 g.5grams14.95485291181.88132.279724.25
10-4410-101.0 g1grams29.91983.67590.2368.88268.27196.7349.18
10-4410-202.0 g2grams59.811980.331188.2742.63540.09396.0799.02

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
10-4410-020.25 g.25grams11.160.07216.8135.598.5513.55
10-4410-050.5 g.5grams22.320.0744027520027.5
10-4410-101.0 g1grams44.630.07886.2553.88402.8255.39
10-4410-202.0 g2grams89.270.0717791111.88808.64111.19


品牌介绍

Glen Research提供了多种荧光团和荧光猝灭剂,可以在寡核苷酸合成过程中的5'-或3'-末端或序列内添加。所得的荧光寡核苷酸探针通常用于多种应用,例如遗传和基因组分析以及法医分析。荧光寡核苷酸探针的常见应用是FRET和分子信标探针。 

生产和包装用于荧光探针设计的Glen Research产品,以确保在商业合成仪上获得最高性能。每批都附有分析报告和HPLC迹线,显示了我们质量控制测试的结果。Glen Research单体包装在行业标准的小瓶中,并经过专门清洁以消除微粒污染。


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