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主营:亚磷酰安,DNA和RNA寡核苷酸的化学合成物
℡ 4000-520-616
℡ 4000-520-616
Glen research/dT-Me Phosphonamidite/1kit/10-1130-02M
产品编号:10-1130-02M
市  场 价:¥4500.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$225.00
品      牌: Glen research
公      司:Glenresearch,inc
公司分类:
Glen research/dT-Me Phosphonamidite/1kit/10-1130-02M
商品介绍
Technical Documents
Description
Details
Dilution/Coupling Data
Related Products

Technical Documents

Safety Data Sheet

Synthesis Using Methyl Phosphoramidites

Glen Report 9.12: Protecting Groups for DNA, RNA and 2"-OMe-RNA Monomers


Description

Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages.However, deprotection and purification techniques differ and a description of the procedures is included in the Technical Bulletin.We also offer the dC monomer with acetyl base protection.1This protecting group is removed with ammonium hydroxide during the cleavage step, eliminating modification at the dC sites during the deprotection step using ethylenediamine in ethanol.

Details

Usage

  • Coupling: 6 minutes. Note to prevent degradation of the methyl phosphonate linkage, low-water content oxidizer (40-4032) and DMAP for Cap B (40-4020) are recommended.
  • Deprotection: See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_Me-Phosphonamidites.pdf).
Specifications
DiluentAnhydrous Acetonitrile
StorageRefrigerated storage, maximum of 2-8°C, dry
Stability24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #Pack SizeGrams/Pack0.1M Dil. (mL)Approximate Number of Additions
LV40LV20040nm0.2μm1μm10μm
10-1130-020.25 g.25grams3.62107.3364.440.2529.2721.475.37
10-1130-050.5 g.5grams7.25228.3313785.6362.2745.6711.42

Expedite

Catalog #Pack SizeGrams/PackDilution (mL)Approximate Number of Additions
Molarity50nm0.2μm1μm15μm
10-1130-020.25 g.25grams5.410.07101.863.6346.276.36
10-1130-050.5 g.5grams10.820.07210131.2595.4513.13


品牌介绍

Glen Research提供了多种荧光团和荧光猝灭剂,可以在寡核苷酸合成过程中的5'-或3'-末端或序列内添加。所得的荧光寡核苷酸探针通常用于多种应用,例如遗传和基因组分析以及法医分析。荧光寡核苷酸探针的常见应用是FRET和分子信标探针。 

生产和包装用于荧光探针设计的Glen Research产品,以确保在商业合成仪上获得最高性能。每批都附有分析报告和HPLC迹线,显示了我们质量控制测试的结果。Glen Research单体包装在行业标准的小瓶中,并经过专门清洁以消除微粒污染。


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