Glen research/5-Ethynyl-dU-CE Phosphoramidite/1kit/10-1554-95,Glen research,,Glen research,Glenresearch,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Glen research/5-Ethynyl-dU-CE Phosphoramidite/1kit/10-1554-95

产品编号：10-1554-95

市场价：¥2600.00

场地：美国(厂家直采)

产品分类：生化试剂>其他>胺>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$130.00

品牌： Glen research

公司：Glenresearch,inc

公司分类：

商品介绍

Technical Documents

Description

Details

Intellectual Property

Dilution/Coupling Data

References

Technical Documents

Safety Data Sheet

Extinction Table

Glen Report 25.24: New Products - TEMPO Spin Labels for Click Chemistry and 5-Ethynyl-dU

Glen Report 26.27: Technical Brief – Use of Click Chemistry for Possible Crosslinking, Click Synthesis of a Potentially Useful azido support and New Click Product - Cyanine 7 Azide

Glen Report 28.22: New Product - TIPS-5-Ethynyl-dU-CE Phosphoramidite

Glen Report 29.15: Sequence Modification using Glen Research"s 5-Modified dU Family

Description

5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat.Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions.Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction.TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection.Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup.A quick treatment with TBAF removes the TIPS protecting group.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: Use ammonium hydroxide and deprotect at room temperature as required by nucleobases. Compatible with UltraMild deprotection. Not compatible with methylamine deprotection.

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30�C, dry
Stability	1-2 days

Intellectual Property

baseclick GmbH has been granted the following patents (1-3) besides its further patent applications (4-5). |1. WO 2006/117161 (New labelling strategies for the sensitive detection of analytes)|2. WO 2008/952775 (Click chemistry for the production of reporter molecules)|3. WO 2010/115957 (Click Chemistry on heterogeneous catalysts)|4. PCT/EP 2013/064610 (Anandamide-modified nucleic molecules)|5. PCT/EP 2015/056007 (Self-assembly of DNA Origami: a diagnostic tool)|baseclick GmbH holds a worldwide exclusive license for granted patent application|WO 03/101972 (Copper-catalysed ligation of azides and acetylenes for the nucleic acid field) in the area of diagnostics and research. As Glen Research and baseclick are partners, Glen Research is now able to help in sublicensing this outstanding technology.

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1554-02	0.25 g	.25grams	3.31	97	58.2	36.38	26.45	19.4	4.85
10-1554-90	100 µmol	.075grams	1	20	12	7.5	5.45	4	1
10-1554-95	50 µmol	.038grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1554-02	0.25 g	.25grams	4.94	0.07	92.4	57.75	42	5.78
10-1554-90	100 µmol	.075grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1554-95	50 µmol	.038grams	0.75	0.07	8.6	5.38	3.91	0.54

References

REFERENCE(S)| 1 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064; V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.| 2 C. J. Burrows, J. G. Muller, Chem. Rev. 1998, 98, 1109 â 1151.| 3 T. R. Chan, R. Hilgraf, K. B. Sharpless, V. V. Fokin, Org. Lett. 2004, 6, 2853 â 2855.| 4 J. Gierlich, G. A. Burley, P. M. E. Gramlich, D. M. Hammond, T. Carell, Org. Lett. 2006, 8, 3639-3642. F. Seela, V. R. Sirivolu, Chem. Biodiversity 2006, 3, 509-514.| 5 P. M. E. Gramlich, S. Warncke, J. Gierlich, T. Carell, Angew. Chem. 2008, 120, 3491â3493; Angew. Chem. Int. Ed. 2008, 47, 3442â 3444.| 6 P. M. E. Gramlich, C. T. Wirges, A. Manetto, T. Carell, Angew. Chem. Int. Ed. 2008, 47, 8350-8358.