The principle of the Glen Research gel filtration column, Glen Gel-Pak™ , is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through The resin of the column. Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.
There are many benefits to Glen Gel-Pak columns:
A User Guide to Glen Gel-Pak™ Purification is available online at: https://www.glenresearch.com/media/productattach/g/l/glen_gel-pak_1.5.2.pdf
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Storage | Controlled room temperature |
Glen Research提供了多种荧光团和荧光猝灭剂,可以在寡核苷酸合成过程中的5'-或3'-末端或序列内添加。所得的荧光寡核苷酸探针通常用于多种应用,例如遗传和基因组分析以及法医分析。荧光寡核苷酸探针的常见应用是FRET和分子信标探针。
生产和包装用于荧光探针设计的Glen Research产品,以确保在商业合成仪上获得最高性能。每批都附有分析报告和HPLC迹线,显示了我们质量控制测试的结果。Glen Research单体包装在行业标准的小瓶中,并经过专门清洁以消除微粒污染。