Glenresearch/dC-H-Phosphonate, DBU Salt/10-1210-05M,Glen research,,Glen research,Glenresearch,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Glenresearch/dC-H-Phosphonate, DBU Salt/10-1210-05M

产品编号：10-1210-05M

市场价：¥1600.00

场地：美国(厂家直采)

产品分类：生化试剂>其他>盐>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$80.00

品牌： Glen research

公司：Glenresearch,inc

公司分类：

商品介绍

Description

Glen Research H-Phosphonates are analyzed by HPLC and are synthesis-tested.H-Phosphonates are especially useful for the preparation of modified internucleotide linkages which are unattainable by phosphoramidite chemistry.The most popular application is the preparation of radiolabelled phosphorothioates, since the sulfurization reaction is carried out off the synthesizer.

Details

Usage

Coupling: H-Phosphonate Synthesis Protocol.|(The DBU is now being provided in place of the TEA salt for dC-H Phosphonate. This product shows better monomer stability and solubility.)
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Acetonitrile/Pyridine (1:1)
Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability	~1week

Technical Documents

Safety Data Sheet

H-Phosphonates

Glen Report 4-11: Introduction

Glen Report 4-12: RNA Synthesis - Options for 2'-0H Protection

Glen Report 4-14: Literature Review

Glen Report 1-13: A Practical Application of Nucleoside H-Phosphonates for Point Modification of Oligonucleotides

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1210-02	0.25 g	.25grams	21	686.67	412	257.5	187.27	137.33	34.33
10-1210-05	0.5 g	.5grams	42	1386.67	832	520	378.18	277.33	69.33

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1210-02	0.25 g	.25grams	5	0.06	93.6	58.5	42.55	5.85
10-1210-05	0.5 g	.5grams	10	0.06	193.6	121	88	12.1

Product FAQs

What are the concentrations of the solutions used on an ABI for H-phosphonate chemistry?
1. Adamantantanecarbonyl chloride - 2% w/v in 5%pyridine/acetonitrile.
2. Isopropyl phosphite - 1% in acetonitrile/pyridine (50:50)
3. Oxidation: Following synthesis
  1. React support with 4% Iodine in THF/Pyridine/Water ( 8:1:1) ~2mL for 4minutes
  2. Remove solution from support
  3. React support with mixture of 4% Iodine in THF/Pyridine/Water ( 8:1:1) (~1mL) and TEA/H2O/THF (8:1:1) (~1mL) for 3 minutes
  4. Flush column with acetonitrile and blow dry support

品牌介绍

Glen Research提供了多种荧光团和荧光猝灭剂，可以在寡核苷酸合成过程中的5'-或3'-末端或序列内添加。所得的荧光寡核苷酸探针通常用于多种应用，例如遗传和基因组分析以及法医分析。荧光寡核苷酸探针的常见应用是FRET和分子信标探针。

生产和包装用于荧光探针设计的Glen Research产品，以确保在商业合成仪上获得最高性能。每批都附有分析报告和HPLC迹线，显示了我们质量控制测试的结果。Glen Research单体包装在行业标准的小瓶中，并经过专门清洁以消除微粒污染。

公司介绍

产品分类

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DNA & RNA Nucleosides, Analogs, and Supports Modification and Labeling Universal Supports and Synthesis Supplies Label Phosphoramidites and Supports Modifier Phosphoramidites and Supports Oligonucleotide Purification Supplies Ancillary Reagents

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