dC when protected as an amide linkage is susceptible to transamination or displacement of the amide with the deprotecting amine.Normally, the deprotecting amine is ammonia and displacement leads to the same product as hydrolysis.However, if methylamine is used for the deprotection step with a benzoyl protected dC, approximately 10% of the product is from the displacement reaction to give N-methyl-dC residues, while the remaining 90% is the desired product formed by hydrolysis.A more labile protecting group on dC is isobutyryl which is also commercially available.This group is displaced by methylamine to about 5% - an improvement but still a mutation.Ac-dC, on the other hand, is hydrolyzed virtually instantaneously by the AMA reagent and none of the slower displacement reaction gets a chance to occur.||REFERENCE(S):M.P. Reddy, Beckman Instruments, personal communication.||

Yes.Store tightly sealed in a glass bottle in the refrigerator.The reagent does not decompose but routine opening will lose some of the volatile bases.Dispose properly after 4-6 weeks.||REFERENCE(S):Candy Lee, Dartmouth